474 Scanning Fluorescence Detector, Waters (CAT#: STEM-CPO-0732-ZJF)

The manufacturer of the 474 Scanning Fluorescence Detector, Waters is Waters.
Waters 474 Scanning Fluorescence Detector brings a new, higher level of sensitivity to HPLC fluorescence detection. When Waters introduced the 470 Scanning Fluorescence Detector, quantitation of naturally fluorescing and derivatized compounds was achieved at levels lower than previously possible by HPLC. That high sensitivity has been surpassed by the Waters 474 Scanning Fluorescence Detector.
Waters 474 Detector optimizes the utility of fluorescence detection with advanced programming and scanning capabilities. Up to 10 programmed methods of 64 program steps each can be stored and automatically executed to change excitation and/or emission wavelengths, detector output gain, or attenuation. This allows optimum detection of multiple analytes in a single run. Excitation and emission spectra can be scanned in a stopped flow mode or on the fly, and the data reviewed post run. The 474 Detector will store up to 10 excitation and emission spectra. The improved electronics in the optics bench minimize baseline noise, which improves sensitivity. For maximum sensitivity, the 474 Detector comes standard with a 16uL flow cell.

Condition: Refurbished, Certified Pre-owned
Monochromator: Holographic concave diffraction grating (for both excitation and emission)
Light source: 150 W Xenon lamp
Wavelength range: 200 to 700 nm (for excitation and emission). Can be set from 0 to 900
Spectrum band width:
Excitation: 18 nm
Emission: 10, 18, or 40 nm (set on keypad)
Wavelength accuracy: ±2 nm
Wavelength repeatability: ±0.3 nm
Detector:
Excitation: Photodiode
Emission: Photomultiplier tube
Second order filter: Automatically inserted into light path when Ex > 400 Nm
Flow cell: 16 µL (standard) or 5 µL
Maximum flow cell pressure:
16 µL: 145 psi (10 bar)
5 µL: 500 psi (34.5 bar)
Wetted surfaces: Synthetic silica, fluororesin, PEEK, stainless steel SXS-316
Sensitivity: S/N for the Raman peak of water is 320 or above, where Ex λ = 350 nm and time constant is 1.5 sec, 16 µL cell (Instruments shipped prior to June 1997, S/N for the Raman peak of water is 200 or above)
Attenuation range (Recorder output): Ten steps, including 1, 2, 4, 8, 16, 32, 64, 128, 256, S
Gain range: 1, 10, 100, 1000
Response: Fast, Standard, Slow, and digital filter
Signal processing: Digital processing by A/D and D/A converters (with surrounding temperature compensation circuits)
Outputs:
Recorder: 10 mV full-scale (can be inverted)
Integrator: 1 V full-scale
Marker output
Leak output
Inputs:
Marker input
Auto Zero input
Program reset
Program functions:
Time program with following variables:
Wavelength change (excitation and emission)
Gain
Attenuation
Wavelength scan
Ex, Em Spectra
Wavelength scan functions: Manual and time program parameters:
Time
Interval
Amplitude
Memory location
Ex, Em Spectra
Spectrum storage: Ten locations for both emission and excitation spectra
Self-test diagnostics:
Memory (ROM and RAM)
DC power
Excitation energy decrease
Leak in cell
Lamp use time
Lamp use timer: Internal timer for accumulating the total hours on the Xenon lamp
Ambient temperature:
10 °C to 32 °C (50 °F to 90 °F) operation
10 °C to 60 °C (50 °F to 140 °F) storage
Relative humidity: 20 to 80%, non-condensing
Optional: Waters 474 performance maintenance kit (p/n WAT052665)

Refurbished to meet manufacturer's specification
In excellent condition
Thoroughly cleaned
Money back guarantee
Certified with warranty

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