Analysis Affinity and Kinetics of Chelated Metals/Metal-Free with 2C12 Monoclonal Antibody by KinExA (CAT#: STEM-MB-0058-CJ)

Introduction

.Chelate, any of a class of coordination or complex compounds consisting of a central metal atom attached to a large molecule, called a ligand, in a cyclic or ring structure. Chelates are more stable than nonchelated compounds of comparable composition, and the more extensive the chelation—that is, the larger the number of ring closures to a metal atom—the more stable the compound. In medical practice, chelating agents, particularly salts of EDTA, or edetic (ethylenediaminetetraacetic) acid, are widely used for direct treatment of metal poisoning because they bind the toxic metal ions more strongly than do the vulnerable components of the living organism. Chelating agents are also employed as extractants in industrial and laboratory separation of metals and as metal-ion buffers and indicators in analytical chemistry. Many commercial dyes and a number of biological substances, including chlorophyll and hemoglobin, are chelate compounds. 2C12 is a monoclonal antibody that can be prepared by BALB/c mouse injection of a Pb (II) -chelate complex covalently coupled to a carrier protein. 2C12 can recognize the Pb (II) -cyclohexyldiethylenetriamine pentaacetic acid complex