Analysis Antibody Binding Affinity and Specificity of Biopharmaceutical Compounds by Enzyme-linked Immunosorbent Assay (ELISA) (CAT#: STEM-B-0387-CJ)

Introduction

Biological activity is, besides the safety of the therapy, the most important aspect of a drug product or therapy. Thus, a major goal of formulation development is to maintain the functionality of the therapeutic compound inside its formulation throughout its shelf-life.

The biologic functions are isotype- and subclass-dependent. In comparison with specificity, affinity of an antibody is a measure of the strength of the binding between antibody and antigen, such that a low-affinity antibody binds weakly and high-affinity antibody binds firmly.