Analysis Biomolecular Interactions of rhDLL1-DE3 Protein with Human Notch1 Recombinant Protein by BLI (CAT#: STEM-MB-0158-CJ)

Introduction

The Notch signalling is an evolutionary conserved pathway that controls a multitude of cellular processes such as stem cell maintenance, cellular differentiation, proliferation, apoptosis, and immune response. Upon ligand binding to Notch receptors, the intracellular domain of the receptor is cleaved and translocated into the nucleus, where it associates to DNA-binding proteins and induces the expression of target genes. In mammals there are five ligands (Jagged1, Jagged2, and Delta-like ligand - DLL1, 3, and 4), each of which can activate any of the four Notch receptors (Notch1-4). In breast cancer, Notch1 and Jagged1 high levels correlate with poor prognosis and poor patient overall survival. The Notch signalling promotes growth, migration, and invasion of breast cancer cells, maintenance of cancer stem cells, and tumor angiogenesis, metastasis, and drug resistance. DLL1 has been reported to contribute to melanoma and choriocarcinoma tumorigenesis. Evidences suggest that DLL1 might play an important role in breast cancer. Immunohistochemical analysis of normal and cancerous tissue revealed DLL1 expression is undetectable in normal breast tissues, but moderate to high in breast cancer.