Analysis Immunoassay of Zearalenone (ZEN) by KinExA (CAT#: STEM-MB-0075-CJ)

Introduction

Zearalenone (ZEN) , also known as RAL and F-2 mycotoxin, is a potent estrogen metabolite produced by certain fusarium and gibberellum species. Zearalenone is a major toxin that binds to estrogen receptors and can cause sterility, miscarriage or other breeding problems. In addition, zearalenone is heat resistant. Its production increases when the climate is warm and the air humidity is 20 percent or more. In addition to its effect on classical estrogen receptors, zearalenone has been found to act as an agonist for GPER (GPR30).

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Principle Applications Procedure Materials Notes Related Products

Principle

KinExA is a two-stage analysis system. In the first stage, a number of solutions are prepared, where one partner remains constant (constant binding partner, or CBP) and the other (titrant) is variable, usually serially diluted. As the titrant is added, the free CBP decreases and is analysed by a sophisticated and precise microfluorescence measurement device. The signal generated can be mathematically related to the affinity (KD) of the two molecules for each other, as well as the kinetic parameters of binding (kon) and dissociation (koff).

Applications

Immunology/Inflammation, Toxicology

Procedure

1. preparation of the functionalized beads which will capture the analyte for measuremen.
2. preparation of a series of solutions consisting of a constant initial concentration of one component of the binary reaction and serial dilutions of the other reactant. The component that is kept constant is the constant binding partner (CBP) , and is the one which will be analyzed.
3. each reaction mixture is sampled and the fluorescence of free CBP bound to the capture beads is obtained for subsequent numerical analysis.

Materials

• Sample Type: Serum, Plasma, Urine, Small Molecules, Lipids
• Equipment: Kinetic Exclusion Assay (KinExA)

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