Inquiry Basket

Analysis Kinetics of Recombinant Human FcγRIIIA/CD16a Receptor by BLI (CAT#: STEM-MB-0275-CJ)

Introduction

Human Fc gamma receptor IIIA (FcγRIIIA, CD16a, V176) His+Avi (Biotinylated) is used to measure the affinity of antibodies for it. therapeutic antibodies. The receptor has been classified based on its affinity for IgG1, IgG3, IgG4.CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, macrophages, and certain T cells.CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which participate in signal transduction.The most well-researched membrane receptor implicated in triggering lysis by NK cells, CD16 is a molecule of the immunoglobulin superfamily (IgSF) involved in antibody-dependent cellular cytotoxicity (ADCC). In humans, CD16 consists of two isoforms, CD16A and CD16B, encoded by two highly homologous genes. CD16A is a transmembrane protein expressed by lymphocytes and some monocytes.

Add to Cart Please Inquire

Principle Applications Procedure Materials

Principle

Bio-Layer Interferometry (BLI) is an optical technique for measuring macromolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. BLI experiments are used to determine the kinetics and affinity of molecular interactions. In a BLI experiment, one molecule is immobilized to a Dip and Read Biosensor and binding to a second molecule is measured. A change in the number of molecules bound to the end of the biosensor tip causes a shift in the interference pattern that is measured in real-time.

Applications

Immunology/Inflammation

Procedure

1. Detect Buffers and prepare samples. BLI experiments are set up with one molecule immobilised on the surface of the biosensor (load sample) and a second molecule in solution (the analytical sample).
2. Fix the load sample on the biocompatible biosensor while the analytical sample is in solution.
3. The biosensor tip is immersed in the solution so that the target molecule begins to bind to the analysis sample.
4. Set up and run the BLI experiment. Molecules bound to or dissociated from the biosensor can generate response curves on the BLI system; unbound molecules, changes in the refractive index of the surrounding medium or changes in flow rate do not affect the interferogram pattern.
5. Collect and analyse data on the BLI's system.

Materials

• Equipment: Fortebio Bio-Layer Interferometry (BLI)
• Sample Type: DNA, RNA, Protein, Antibodies, Peptides, Small Molecules
• Optionals: Infliximab, 2-Hydroxypropyl-beta-cyclodextrin, Sodium-phosphate Buffer

Other recommended products

Analysis Biomolecular Interactions of IgG with Z33 Analog by BLI (CAT#: STEM-MB-0182-CJ)
Analysis Biomolecular Interactions of Nluc-ch2C5 and HRP-ch2C5 to S-RBD Protein by BLI (CAT#: STEM-MB-0170-CJ)
Analysis Biomolecular Interactions of EINsc, Rsdec or HPrK/Pbs to HPr38, HPr59 and HPr70 by BLI (CAT#: STEM-MB-0165-CJ)
Analysis Biomolecular Interactions of COVA309 mAbs with Omicron BA.1 and BA.2 by BLI (CAT#: STEM-MB-0174-CJ)
Analysis Biomolecular Interactions of Pfs25 Monoclonal Antibodies (mAbs) to Pfs28 by BLI (CAT#: STEM-MB-0166-CJ)
Analysis Biomolecular Interactions of TREM2 with ApoE by BLI (CAT#: STEM-MB-0236-CJ)
Analysis Biomolecular Interactions of Monoclonal Antibodies with Nonspecifically with Polyclonal Antibodies by BLI (CAT#: STEM-MB-0128-CJ)
Analysis Kinetics of gH/gL-Targeting Antibodies by BLI (CAT#: STEM-MB-0289-CJ)

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Accept Cookies