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Analysis of AGP (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0014-LGZ)

Introduction

α1-acidglycoprotein (α1-acidglycoprotein, AGP or AAG), called serum mucoid (ORM) in the early stage, is a non-specific acute reaction protein, It is also the glycoprotein with the most sugar content (about 45% sugar content) and the most acidic (PI 2.7 ~ 3.5) in human serum. It is mainly produced by liver macrophages and granulocytes. Later studies have found that cancer cells can also be synthesized. AGP serum concentrations can be affected by certain physiological and pathological conditions, such as pregnancy, burns, certain medications, and specific diseases, especially HIV infection. With the increase of age, AGP serum concentration also decreased.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Metabolic, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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