Analysis of AIP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2450-LGZ)

Introduction

Official Full Name: aryl hydrocarbon receptor interacting protein<br />Also known as: ARA9; XAP2; PITA1; XAP-2; FKBP16; FKBP37; SMTPHN<br />The protein encoded by this gene is an aryl hydrocarbon receptor and a ligand-activated transcription factor. The encoded protein is present in the cytoplasm as part of a multiprotein complex, but is transported into the nucleus after binding to a ligand. This protein can regulate the expression of various exogenous metabolic enzymes. In addition, the encoded protein can specifically bind and inhibit the activity of hepatitis B virus. Three transcript variants encoding different isoforms have been found for this gene.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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