Analysis of ALPP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1769-LGZ)

Introduction

Official Full Name: alkaline phosphatase, placental
Also known as: ALP; IAP; ALPI; PALP; PLAP; PLAP-1
The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphate monoesters. It belongs to a multigene family consisting of four alkaline phosphatase isozymes. The enzyme functions as a homodimer whose catalytic site contains one magnesium ion and two zinc ions, which are required for its enzymatic function. One of the main sources of this enzyme is the liver, therefore, it is one of several indicators of liver damage in different clinical conditions. In pregnant women, this protein is mainly expressed in placental and endometrial tissues, but strong ectopic expression is also found in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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