Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of Antithrombin-III / Serpin C1 / ATIII by ELISA (CAT#: STEM-MB-1557-LGZ)

Introduction

Antithrombin-III, a member of the Serpin superfamily encoded by the SerpinC1 gene, has been found to be a marker of disseminated intravascular coagulation (DIC). This protein inhibits thrombin and other activated serine proteases from the clotting system and regulates the clotting cascade. Antithrombin-III is the most important thrombin factor inhibitor, and even small changes to Antithrombin-III significantly increase the risk of thromembolism. Antithrombin-III can inhibit inflammation by either dependent or independent thrombin effects. In addition to Antithrombin-III deficiency, Antithrombin-III and its encoded gene SerpinC1 may also be associated with many diseases, such as hypertension and kidney disease.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
Advertisement