Analysis of ARID3C Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2053-LGZ)

Introduction

Official Full Name: AT-rich interaction domain 3C<br />This gene is a member of the ARID (AT-rich interaction domain) family of proteins. The ARID domain is a helix-turn-helix motif-based DNA-binding domain. ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation and possibly in chromatin structure modification.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of NDUFA10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1658-LGZ)
Analysis of Clostridium Equi by Real-Time PCR Method (CAT#: STEM-MB-2641-LGZ)
Analysis of SLAMF7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0655-LGZ)
Analysis of Clostridium Septicum by Real-Time PCR Method (CAT#: STEM-MB-3436-LGZ)
Analysis of Pasteurella Pneumotropica by Real-Time PCR Method (CAT#: STEM-MB-3625-LGZ)
Analysis of RPL11 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0089-LGZ)
Analysis of Cowpox Virus (CPV) by Real-Time PCR Method (CAT#: STEM-MB-2616-LGZ)
Analysis of TPRG1L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1441-LGZ)