Analysis of ATP13A2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0083-LGZ)

Introduction

Also known as: CLN12; KRPPD; PARK9; SPG78; HSA9947<br />This gene encodes a member of the P5 subfamily of ATPases which transports inorganic cations as well as other substrates. Mutations in this gene are associated with Kufor-Rakeb syndrome (KRS), also referred to as Parkinson disease 9. Multiple transcript variants encoding different isoforms have been found for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Autosomal recessive spastic paraplegia type 78, Kufor-Rakeb syndrome, Kufor-Rakeb syndrome

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Plasmodium Knowlesi by Real-Time PCR Method (CAT#: STEM-MB-3243-LGZ)
Analysis of Cladosporium Spp. by Real-Time PCR Method (CAT#: STEM-MB-2653-LGZ)
Analysis of RORC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0642-LGZ)
Analysis of PAX7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0085-LGZ)
Analysis of MAB21L4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1641-LGZ)
Analysis of Gordil Virus by Real-Time PCR Method (CAT#: STEM-MB-3594-LGZ)
Analysis of Vesicular Exanthema of Swine Virus (VESV) by Real-Time PCR Method (CAT#: STEM-MB-3196-LGZ)
Analysis of OR51G1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2329-LGZ)