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Analysis of ATP6V1B2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1923-LGZ)

Introduction

Official Full Name: ATPase H+ transporting V1 subunit B2<br />Also known as: DOOD; HO57; VATB; VPP3; Vma2; ZLS2; ATP6B2; ATP6B1B2<br />This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles in eukaryotic cells. V-ATPase-dependent acidification of organelles is essential for intracellular processes such as protein sorting, zymogen activation, receptor-mediated endocytosis, and generation of synaptic vesicle proton gradients. V-ATPase consists of a cytoplasmic V1 domain and a transmembrane V0 domain. The V1 domain consists of 3 A, 3 B and 2 G subunits and C, D, E, F and H subunits. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of the two V1 domain B subunit subtypes and the only B subtype highly expressed in osteoclasts.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements