Analysis of B3GALT2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0172-LGZ)

Introduction

Also known as: GLCT2; BETA3GALT2; beta3Gal-T2
This gene is a member of the β-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with distinct enzymatic functions using different donor substrates (udp-galactose and udp-n-acetylglucosamine) and different acceptor sugars (n-acetylglucosamine, galactose, n-acetylglucosamine). The beta3GalT gene is distantly related to the Drosophila Brainiac gene, and its protein-coding sequence is contained in a single exon. The beta3GalT protein also contains conserved sequences not found in the beta4GalT or alpha3GalT proteins. Carbohydrate chains synthesized by these enzymes are assigned type 1, whereas those synthesized by β-4galt enzymes are type 2. The ratio of type 1 and type 2 strands changes during embryogenesis. Based on sequence similarity, the beta3GalT genes are divided into at least two groups: beta3GalT4 and four other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene encodes a protein that functions in the glycosylation of N-linked glycoproteins and exhibits strict donor substrate specificity for UDP-galactose.