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Analysis of Bacillus Anthraci by Real-Time PCR Method (CAT#: STEM-MB-2880-LGZ)

Introduction

A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time),not at its end,as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter,which permits detection only after hybridization of the probe with its complementary sequence.




Principle

In this study, specific primers and probes were designed for highly conserved regions of LSDV. PCR reaction was carried out and fluorescence signals were released when the LSDV genomic template was included in the reaction system. The signal strength of the corresponding channel in the PCR process was monitored and output in real time by the instrument, and the qualitative analysis of the detection results was realized.

Applications

It can be used for qualitative detection of LSDV and has important guiding significance for diagnosis and monitoring of bovine blight caused by LSDV.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

• Sample Type: Body fluids/tissues/blood samples of infected animals