Analysis of BMP-5 (Human) by ELISA (CAT#: STEM-MB-0700-LGZ)

Introduction

Bone morphogenetic protein (BMP), also known as bone morphogenic protein, is a group of highly conserved functional proteins with similar structures, belonging to the TGF-β family. BMP can stimulate DNA synthesis and cell replication, thereby promoting the directional differentiation of mesenchymal cells into osteoblasts.<br />BMP-5 is expressed in the nervous system, lung and liver. It is a regulator of dendritic cell growth in sympathetic neurons. BMP-5 is a 454 amino acid precursor protein that is cleaved to release the biologically active C-terminal mature protein.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Bone Disorders

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

Other recommended products

Analysis of BCMA/TNFRSF17 (Human) by ELISA (CAT#: STEM-MB-0693-LGZ)
Analysis of IgM (Mouse) by ELISA (CAT#: STEM-MB-0926-LGZ)
Analysis of Plant Amino Carboxylic Acid (ACC) by ELISA (CAT#: STEM-MB-2280-LGZ)
Analysis of Plant Gibberellic Acid 20 (GA20) by ELISA (CAT#: STEM-MB-2264-LGZ)
Analysis of Human SP-D(Pulmonary surfactant-associated protein D) by ELISA (CAT#: STEM-MB-1765-LGZ)
Analysis of Plant a-Linolenic acid (a-LA) by ELISA (CAT#: STEM-MB-2391-LGZ)
Analysis of MIC-A (Human) by ELISA (CAT#: STEM-MB-1051-LGZ)
Analysis of Human KL(Klotho) by ELISA (CAT#: STEM-MB-1895-LGZ)