Analysis of CARS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2212-LGZ)

Introduction

Official Full Name: cysteinyl-tRNA synthetase 1<br />Also known as: CARS; MDBH; CYSRS; MCDDBH; MGC:11246<br />This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteine-tRNA synthetase. Each of the 20 aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isofamily with cognate amino acids. The gene is located near the imprinted gene region of chromosome 11p15.5, which is an important tumor suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and cancers of the lung, ovary, and breast. Alternative splicing of this gene results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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