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Analysis of CCNH Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1845-LGZ)

Introduction

Official Full Name: cyclin H<br />Also known as: CAK; p34; p37; CycH<br />The protein encoded by this gene belongs to the highly conserved cyclin family, members of which are characterized by a pronounced periodicity in protein abundance through the cell cycle. Cyclins are regulators of CDK kinases. Different cyclins exhibit distinct patterns of expression and degradation, which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with the CDK7 kinase and the ring finger protein MAT1. This kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thereby functioning as a cdk-activating kinase (CAK). This cyclin and its kinase partner are part of the TFIIH and RNA polymerase II protein complex. They are involved in two distinct transcriptional regulatory processes, suggesting an important link between underlying transcriptional control and cell cycle machinery. A pseudogene of this gene has been found on chromosome 4. Alternate splicing results in multiple transcript variants.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements