Analysis of CCT3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0833-LGZ)

Introduction

Official Full Name: chaperonin containing TCP1 subunit 3<br />Also known as: CCTG; PIG48; TRIC5; CCT-gamma; TCP-1-gamma<br />The protein encoded by this gene is a molecular chaperone, a member of the chaperone containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. This complex folds various proteins, including actin and tubulin. Additional transcriptional splice variants of this gene have been characterized. In addition, a pseudogene of this gene was found on chromosome 8.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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