Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of CD276/B7-H3 (Human) by ELISA (CAT#: STEM-MB-0723-LGZ)

Introduction

B7-H3 (B7 homolog 3 protein), also known as CD276, is an important immune checkpoint molecule of the B7-CD28 family. It is a member of the B7 family identified in the human dendritic cell cDNA library by Chapoval et al. in 2001. B7-H3 mainly exists in membrane protein and soluble form, and soluble B7-H3 (soluble B7-H3, sB7-H3) is cleaved from membrane protein by metalloprotease.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or cell culture supernatant
Advertisement