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Analysis of CD30/TNFRSF8 (Human) by ELISA (CAT#: STEM-MB-0725-LGZ)

Introduction

Leukocyte antigen cluster of differentiation 30 (CD30), also known as tumor necrosis factor receptor superfamily member 8 (TNFRSF8), is a membrane protein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. The CD30 protein is expressed by activated but not resting T cells and B cells. CD30 may play a role in the regulation of cell growth and transformation of activated lymphoblastic cells, and may also play an important role in human immunodeficiency virus replication. TRAF2 and TRAF5 can interact with this receptor and mediate signal transduction leading to NF-κB activation. As a positive regulator of apoptosis, the CD30 protein can induce cell death or proliferation depending on the cell type and has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body from autoimmunity. CD30 protein expression is upregulated in various hematological malignancies, and CD30 is also associated with leukocytes in patients with chronic inflammatory diseases such as lupus, asthma, and rheumatoid arthritis.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or cell culture supernatant
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