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Analysis of CK Beta 8-1/CCL23 (Human) by ELISA (CAT#: STEM-MB-0746-LGZ)

Introduction

Human Chemokine (C-C Motif) Ligand 23 (CCL23) is a small cytokine belonging to the CC chemokine family. CCL23 is also known as myeloid progenitor inhibitory factor MPIF-1, CK8 and SCYA23. The CCL23 cDNA encodes a 120 amino acid residue precursor protein containing a 21 amino acid residue signal peptide, which is cleaved to generate a 99 amino acid residue mature CCL23 (amino acids 22-120). Additional N-terminal processing of the 99 amino acid residue variant produced a 75 amino acid residue peptide (amino acids 46-120) that was significantly more active than the 99 amino acid residue variant. CCL23 has high affinity to CCR1 and has chemotactic activity on monocytes, dendritic cells and osteoclast precursors. CCL23 enhances angiogenesis of endothelial cells but reduces proliferation of progenitor cells giving rise to granulocytic and monocyte lineages.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: serum, plasma, supernatant
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