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Analysis of CLK2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0861-LGZ)

Introduction

Official Full Name: CDC like kinase 2
This gene encodes a dual-specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. The activity of this protein regulates the serine- and arginine-rich (SR) proteins of the spliceosome complex, thereby affecting alternative splicing of transcripts. A chromosomal translocation occurs between this site and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias but encodes a protein involved in the regulation of telomere length. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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