Analysis of Contactin-2/TAG-1 (Human) by ELISA (CAT#: STEM-MB-0755-LGZ)

Introduction

Contactin 2 (CNTN2, transiently-expressed axonal glycoprotein, AXT, TAX, TAX1, TAG-1, axonin 1) is a neuronal cell adhesion molecule (CAM) that influences the formation of axon connections in the developing nervous system. Contactin 2 is a member of the immunoglobulin superfamily (IgSF) and contains glycosyl phosphatidylinositol anchors, six immunoglobulin (Ig) -like domains, and four type III fibronectin (FNIII) -like domains. Contactin 2 is expressed mainly on axon cell membranes in nerve fiber bundles during neural development to guide the junction axon without promoting its growth. Contactin 2 binds to NgCAM in the same membrane plane (cis-binding). Contactin 2 heterotropy (Contactin 2/NgCAM and Contactin 2/NrCAM) binding sites were located in the first four Ig domains. The Contactin 2 homology (Contactin 2/Contactin 2) binding site is located in the FNIII domain.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Alzheimer’s Disease, Neurobiology/Neurodegeneration

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: serum, plasma, supernatant

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