Analysis of Dekkera Bruxellensis by Real-Time PCR Method (CAT#: STEM-MB-3667-LGZ)

Introduction

Dekkera bruxellensis are spoilage yeasts associated with wine and to a lesser extent other beverages. Its teleomorph is Brettanomyces, and these yeasts are commonly known as the ‘Brett’ yeasts in the wine industry, where they cause an undesirable spoilage of maturing wine.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.