Analysis of DF3 by UV-Vis Spectroscopy (CAT#: STEM-MB-0973-WXH)

Introduction

DF3 is the latest member of the DF family of synthetic proteins. They consist of helix–loop–helix hairpins, designed to dimerize and form an antiparallel four-helix bundle that encompasses a metal-binding site similar to those of non-heme carboxylate-bridged di-iron proteins. Unlike previous DF proteins, DF3 is highly soluble in water (up to 3 mM) and forms stable complexes with several metal ions (Zn, Co, and Mn), with the desired secondary structure and the expected stoichiometry of two ions per protein.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer

Other recommended products

Characterization of papain conformation in sol-gel by UV-Vis Spectroscopy (CAT#: STEM-MB-0892-WXH)
Analysis of protein structures by UV-Vis Spectroscopy (CAT#: STEM-MB-0944-WXH)
Identification of the cytotoxicity of superparamagnetic iron oxide nanoparticles by UV-Vis Spectroscopy (CAT#: STEM-MB-0919-WXH)
Blood characterization by UV-Vis Spectroscopy (CAT#: STEM-MB-0904-WXH)
Analysis of human serum albumin (HSA) Conjugation with Gold Nanoparticles by UV-Vis Spectroscopy (CAT#: STEM-MB-0975-WXH)
Analysis of structural changes in putidaredoxin by UV-Vis Spectroscopy (CAT#: STEM-MB-0907-WXH)
Analysis of Thermal Denaturation of Proteins by UV-Vis Spectroscopy (CAT#: STEM-MB-0950-WXH)
Analysis of interaction of silver nanoparticles-protein by UV-Vis Spectroscopy (CAT#: STEM-MB-0968-WXH)