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Analysis of Eotaxin-3 (Human) by ELISA (CAT#: STEM-MB-0788-LGZ)

Introduction

Eotaxin is a potent chemoattractant for eosinophils. It is a member of the CC chemokine family and it is expressed only in the adult gut, thymus and skin. The human factor consists of 74 amino acid residues. Its members are produced by lung and bronchial epithelial cells, induced by interleukin-4, and repressed by interferon gamma. Mainly responsible for the infiltration of eosinophils into the site of allergic inflammation.
Eotaxin-3 has a chemotactic effect on eosinophils and basophils, and exerts its effect by binding to the cell surface chemokine receptor CCR3. It is expressed in multiple tissues including heart, lung and ovary, and in endothelial cells stimulated by the cytokine interleukin 4. The chemokine gene is located on human chromosome 7.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids
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