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Analysis of ErbB3/HER3 (Human) by ELISA (CAT#: STEM-MB-0795-LGZ)

Introduction

Tyrosine kinase receptor ErbB3, also known as human epidermal growth factor receptor 3 (HER3), is a membrane-bound protein encoded by the ERBB3 gene in humans. ErbB3 is a member of the epidermal growth factor receptor family (EGFR/ERBB), and like other EGFR family members, it consists of three parts: an extracellular ligand-binding domain, a transmembrane domain and an intracellular domain. ErbB3 binds neuregulin ligands, but it cannot mediate intracellular signaling through protein phosphorylation. However, the ErbB3 heterodimer responsible for tyrosine phosphorylation can form a receptor complex with high affinity with ErbB2 or other EGFR members, and can also initiate related pathways of cell proliferation or differentiation. ErbB3 has been shown to be related to many Malignant tumors, such as prostate, bladder and breast tumors.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, Hep Plasma, Serum
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