Analysis of FMN2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0969-LGZ)

Introduction

Official Full Name: formin 2
This gene is a member of the biguanide homolog protein family. The encoded protein is thought to play an important role in the organization and cellular polarity of the actin cytoskeleton. This protein mediates the formation of actin meshwork, positions the spindle during oogenesis, and also regulates the formation of actin filaments in the nucleus. This protein also forms the perinuclear actin/focal adhesion system, which regulates the shape and position of the nucleus during cell migration. Mutations in this gene are associated with infertility and an autosomal recessive form of intellectual disability (MRT47). Another spliced transcript variant has been identified.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Moraxella Catarrhalis by Real-Time PCR Method (CAT#: STEM-MB-3792-LGZ)
Analysis of Coxsackie Virus (CA24v) by Real-Time PCR Method (CAT#: STEM-MB-3090-LGZ)
Analysis of STIM1-AS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2160-LGZ)
Analysis of Swine Papillomavirus by Real-Time PCR Method (CAT#: STEM-MB-3207-LGZ)
Analysis of MIR126 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2000-LGZ)
Analysis of Mycobacterium Microti by Real-Time PCR Method (CAT#: STEM-MB-3565-LGZ)
Analysis of Bacillus Anthraci by Real-Time PCR Method (CAT#: STEM-MB-2880-LGZ)
Analysis of FALEC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0945-LGZ)