Analysis of G-protein-coupled receptors by Fluorescence recovery after photobleaching (FRAP) (CAT#: STEM-MT-0038-WXH)

Introduction

G protein-coupled receptor (GPCR), also called seven-transmembrane receptor or heptahelical receptor, protein located in the cell membrane that binds extracellular substances and transmits signals from these substances to an intracellular molecule called a G protein (guanine nucleotide-binding protein). GPCRs are found in the cell membranes of a wide range of organisms, including mammals, plants, microorganisms, and invertebrates. There are numerous different types of GPCRs—some 1,000 types are encoded by the human genome alone—and as a group they respond to a diverse range of substances, including light, hormones, amines, neurotransmitters, and lipids.