Analysis of GDF2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2078-LGZ)

Introduction

Official Full Name: growth differentiation factor 2
Also known as: BMP9; HHT5; BMP-9
This gene encodes a secreted ligand for the tgf-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-β receptors, leading to the recruitment and activation of SMAD family transcription factors, which regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of a disulfide-linked homodimer. This protein regulates cartilage and skeletal development, angiogenesis, and differentiation of cholinergic CNS neurons. Mutations in this gene are associated with hereditary hemorrhagic telangiectasia.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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