Analysis of GPR35 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1560-LGZ)

Introduction

Official Full Name: G protein-coupled receptor 35<br />Enables C-X-C chemokine receptor activity. Involved in several processes, including chemokine-mediated signaling pathway; negative regulation of voltage-gated calcium channel activity; and positive regulation of cytosolic calcium ion concentration. Is integral component of plasma membrane.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Rinderpest Virus by Real-Time PCR Method (CAT#: STEM-MB-2881-LGZ)
Analysis of GJC2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0986-LGZ)
Analysis of HBB-HS-107 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2242-LGZ)
Analysis of MIR215 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0446-LGZ)
Analysis of PRKCZ Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1398-LGZ)
Analysis of SLAMF9 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0653-LGZ)
Analysis of Salmonella Typhimurium by Real-Time PCR Method (CAT#: STEM-MB-3410-LGZ)
Analysis of FMDA Ag by Real-Time PCR Method (CAT#: STEM-MB-2916-LGZ)