Analysis of H19 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2245-LGZ)

Introduction

Official Full Name: H19 imprinted maternally expressed transcript
Also known as: ASM; BWS; WT2; ASM1; D11S813E; MIR675HG; LINC00008; NCRNA00008
The gene is located in the imprinted region of chromosome 11 next to the insulin-like growth factor 2 (IGF2) gene. The gene is expressed only on maternally inherited chromosomes, while IGF2 is only expressed on paternally inherited chromosomes. The product of this gene is a long non-coding RNA with tumor suppressor effect. Mutations in this gene have been associated with Beckwith syndrome and Wilms tumorigenesis. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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