Analysis of HS-E1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2235-LGZ)

Introduction

Gene description: beta-globin hypersensitive site-embryonic 1<br />Gene type: biological region<br />This genomic region represents a DNase I hypersensitive site that is located in the embryonic gene region of the beta-globin gene cluster, between the epsilon (HBE1) and G-gamma (HBG2) genes. The orthologous hypersensitive site in mouse functions as an embryonic stage-specific enhancer in erythroid cells, and is necessary for active histone acetylation patterns in the embryonic domain.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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