Analysis of HSPD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1505-LGZ)

Introduction

Official Full Name: heat shock protein family D (Hsp60) member 1<br />Also known as: HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60<br />This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Hereditary spastic paraplegia 13

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of ZBTB17 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1430-LGZ)
Analysis of AJM1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2014-LGZ)
Analysis of NUP210L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0212-LGZ)
Analysis of AURKAIP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1240-LGZ)
Analysis of Sabia Virus (SABV) by Real-Time PCR Method (CAT#: STEM-MB-3854-LGZ)
Analysis of PRAMEF20 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1395-LGZ)
Analysis of Sporothrix Schenckii by Real-Time PCR Method (CAT#: STEM-MB-3850-LGZ)
Analysis of RAB11FIP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1890-LGZ)