Analysis of Human T-cell Lymphoma Virus 1 (HTLV-1) by Real-Time PCR Method (CAT#: STEM-MB-2878-LGZ)

Introduction

A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time),not at its end,as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter,which permits detection only after hybridization of the probe with its complementary sequence.

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Principle Applications Procedure Materials Notes Related Products

Principle

Humant-lymphotropic virus type 1 is an RNA virus that causes blood and other metastatic cancers after infection, Infection mainly with CD4+T lymphocytes in the body can lead to adult T-lymphocytic leukemia/lymphoma, HTLV-associated myelopathy (HAM/ tropical spastic palsy) and so on. Tropicalspasticparaparesis (TSP) and pigment phlogistic (uveitis), mainly through blood transfusions, needle, sex and mother-to-child transmission. HTLV-I is a distant relative of HIV (HIV-I), but it has not been linked to the development of AIDS.
Primers and probes involved in this experiment have been optimized with high sensitivity. The primer is highly specific and is designed according to the highly conserved region of HTLV virus type 1. It will not cross-react with RNA of other viruses.

Applications

It can be used for qualitative detection and quantitative detection. The linear range is at least 5 orders of magnitude when used for quantitative detection.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

• Sample Type: serum

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