Analysis of IL-1β (Mouse) by ELISA (CAT#: STEM-MB-0963-LGZ)

Introduction

Interleukin-1 (IL-1) is a cytokine in the chemokine family. A total of 38 interleukins have been found, named IL-1---IL38. They have complex functions. Interleukin-1 is also known as lymphocyte stimulating factor. Mainly produced by activated monocyte-macrophages. The main forms are IL-1α and IL-1β, which can synergistically stimulate the activation of APC and T cells, promote the proliferation of B cells and secrete antibodies, induce the synthesis of proteins in the acute phase of the liver, and cause fever and cachexia.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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