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Analysis of IL-8 (Human) by ELISA (CAT#: STEM-MB-0999-LGZ)

Introduction

Interleukin-8(IL-8), a cytokine in the chemokine family, is known to play a role in regulating the physiological and pathological processes of human reproduction, and one of its mechanisms of action is binding to its specific receptors. It mainly mediates the immune response related to cytotoxicity and local inflammation to assist the generation of antibodies, and participates in cellular immunity and the occurrence of delayed hypersensitive inflammation. The main biological effect is to chemotaxis and activate neutrophils, and promote the lysosomal activity and phagocytosis of neutrophils.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids