Analysis of IL20 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1069-LGZ)

Introduction

Official Full Name: interleukin 20<br />Also known as: IL-20; IL10D; ZCYTO10<br />The protein encoded by this gene is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of OR14C36 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0513-LGZ)
Analysis of MRPL23 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2308-LGZ)
Analysis of ZBTB17 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1430-LGZ)
Analysis of Abalone Shriveling Syndrome-associated Virus (AbSV) by Real-Time PCR Method (CAT#: STEM-MB-3650-LGZ)
Analysis of LIPJ Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2086-LGZ)
Analysis of Hepatitis B Virus(HBV) - TypeA by Real-Time PCR Method (CAT#: STEM-MB-3473-LGZ)
Analysis of SMG7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0311-LGZ)
Analysis of MYD88L265P Mutation by RT-qPCR (CAT#: STEM-MT-0051-LGZ)