Analysis of ILF2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1065-LGZ)

Introduction

Official Full Name: interleukin enhancer binding factor 2
Also known as: NF45; PRO3063
The protein encoded by this gene is a transcription factor required for the expression of the interleukin 2 gene by T cells. It also binds RNA and is an essential component of the encapsidation and protein priming of the hepatitis B virus polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein may delay cell growth by inhibiting mRNA stabilization. Alternative splicing results in multiple transcript variants. Associated pseudogenes were found on chromosomes 3 and 14.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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