Analysis of ISG15 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1344-LGZ)

Introduction

Official Full Name: ISG15 ubiquitin like modifier
Also known as: G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
The protein encoded by this gene is a ubiquitin-like protein that binds to intracellular target proteins upon activation by interferon-α and interferon-β. The encoded protein has multiple functions, including chemotactic activity on neutrophils, orientation of linking target proteins to intermediate filaments, intercellular signaling, and antiviral activity during viral infection. Although conjugates of this protein have been found to attach non-covalently to intermediate filaments, the protein is sometimes secreted.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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