Analysis of KCNH1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1083-LGZ)

Introduction

Official Full Name: potassium voltage-gated channel subfamily H member 1
Also known as: EAG; EAG1; ZLS1; hEAG; TMBTS; h-eag; hEAG1; Kv10.1
Voltage-gated potassium channels are the most complex class of voltage-gated ion channels from both functional and structural points of view. Their diverse functions include regulation of neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the h subfamily of voltage-gated potassium channels, which is the pore-forming (α) subunit of voltage-gated non-inactivating delayed-rectifier potassium channels. It is activated at the onset of myoblast differentiation. This gene is highly expressed in brain and myoblasts. Overexpression of this gene may give cancer cells a growth advantage and favor tumor cell proliferation. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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