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Analysis of KDM5B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0342-LGZ)

Introduction

Official Full Name: lysine demethylase 5B
Also known as: CT31; PLU1; PUT1; MRT65; PLU-1; JARID1B; PPP1R98; RBP2-H1; RBBP2H1A
This gene encodes a lysine-specific histone demethylase and belongs to the jumonji/ARID domain-containing histone demethylase family. The encoded protein is capable of demethylating trimethyl, dimethyl and monomethyllysine 4 of histone H3. This protein plays a role in the transcriptional repression of certain tumor suppressor genes and is upregulated in some cancer cells. The protein may also play a role in genome stabilization and DNA repair. Alternate splicing results in multiple transcript variants.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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