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Analysis of LIGHT/TNFSF14 (Human) by ELISA (CAT#: STEM-MB-1023-LGZ)

Introduction

Tumor necrosis factor ligand superfamily member 14 (TNFSF14), also known as LIGHT, is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediators as well as decoy receptor 3. This protein is a ligand for TNFRSF14, a member of the tumor necrosis factor receptor superfamily also known as a herpesvirus entry medium (HVEM). It has two alternatively spliced transcript variants encoding different isoforms. It acts as a co-stimulator for the activation of lymphocytes and as a deterrent for herpes virus infection. It has been shown to stimulate the proliferation of T cells, induce apoptosis of various tumor cells and play a role in the normalization of blood vessels. It also prevented TNF-α-mediated apoptosis in primary hepatocytes. It has been shown to interact with TNFRSF14, TNFRSF6B, BIRC2, TRAF2 and TRAF3.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or supernatant
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