Analysis of LOC107305685 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1461-LGZ)

Introduction

Gene description: 2q12.3 distal recombination region<br />This region has been shown to undergo non-allelic homologous recombination (NAHR) with a similar low-copy repeat region, the 2q13 recombination region, located about 1.3 Mb downstream of this region, in direct orientation on the reference genome. Deletion of the intervening sequence as a result of NAHR between these recombination regions has been observed. This region overlaps an Alu element.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of GALM Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1739-LGZ)
Analysis of LIPK Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2085-LGZ)
Analysis of OR10Z1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0152-LGZ)
Analysis of MRPL23-AS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2307-LGZ)
Analysis of Rabies Virus (RV、street virus) by Real-Time PCR Method (CAT#: STEM-MB-3107-LGZ)
Analysis of TAFA3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1142-LGZ)
Analysis of Campylobacter Coli by Real-Time PCR Method (CAT#: STEM-MB-2714-LGZ)
Analysis of PGLYRP3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0580-LGZ)