Analysis of MCP-5/CCL12 (Human) by ELISA (CAT#: STEM-MB-1042-LGZ)

Introduction

CCL12, also known as monocyte chemoattractant protein 5 (MCP-5), belongs to the CC chemokine cluster on mouse chromosome 11. Because it is structurally closest to human MCP-1 (66% amino acid identity), it is sometimes referred to as an MCP-1-related chemokine. CCL12 is a potent chemoattractant for peripheral blood monocytes, a chemokine that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Induces calcium flux in peripheral blood mononuclear cells but not in purified mouse eosinophils or neutrophils. CCL12 is a potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and host response to pathogens, and may play a key role in the early stages of allergic lung inflammation.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Amniotic fluid, plasma, serum, supernatant

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