Analysis of MET (soluble) (Human) by ELISA (CAT#: STEM-MB-1050-LGZ)

Introduction

c-Met (cellular-mesenchymal to epithelial transition factor) gene, also known as MET gene, is a proto-oncogene, transmembrane receptor encoding hepatocyte growth factor, c-Met possesses tyrosine kinase (TKs) activity, which is the only known high affinity receptor for hepatocyte growth factor. Activated c-Met binds to hepatocyte growth factor (HGF) to regulate the proliferation, differentiation and invasion activities of various tissues and cells in the body. Once the constituted HGF/c-Met signaling pathway is abnormally activated, it will promote the occurrence, development and metastasis of various tumors.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: plasma, serum, supernatant

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