Analysis of NAP1L4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2301-LGZ)

Introduction

Official Full Name: nucleosome assembly protein 1 like 4
Also known as: NAP2; NAP2L; hNAP2; NAP1L4b
This gene encodes a member of the nucleosome assembly protein (NAP) family that can interact with core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. The gene is one of several located near the imprinted gene region of 11p15.5, an important tumor suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and cancers of the lung, ovary, and breast.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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