Analysis of NAV1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0379-LGZ)

Introduction

Official Full Name: neuron navigator 1
Also known as: POMFIL3; UNC53H1; STEERIN1
This gene belongs to the neuron navigator family and is expressed predominantly in the nervous system. The encoded protein contains coiled-coil domains and a conserved AAA domain characteristic for ATPases associated with a variety of cellular activities. This gene is similar to unc-53, a Caenorhabditis elegans gene involved in axon guidance. The exact function of this gene is not known, but it is thought to play a role in in neuronal development and regeneration. Alternative splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Detection of Point Mutations in KRAS Oncogene by RT-qPCR (CAT#: STEM-MT-0030-LGZ)
Analysis of Mannheimia Haemolytica by Real-Time PCR Method (CAT#: STEM-MB-3534-LGZ)
Analysis of PYCR2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0605-LGZ)
Analysis of F11R Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0946-LGZ)
Analysis of Enterovirus Type 70 (EV70) by Real-Time PCR Method (CAT#: STEM-MB-3905-LGZ)
Analysis of Amycolata Autotrophica by Real-Time PCR Method (CAT#: STEM-MB-3437-LGZ)
Analysis of IL24 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1068-LGZ)
Analysis of CD81-AS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2205-LGZ)