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Analysis of Nutraceuticals by Near Infrared (NIR) Spectroscopy (CAT#: STEM-ST-1504-CJ)

Introduction

The term “nutraceuticals” has been introduced in 1989 referring to food or part of food that can be of plant or animal origin to exert a valuable pharmaceutical activity in addition to its nutritional value. For instance, green tea is recognized for its anti-obesity and cardioprotective effects, while garlic is used for its antihypertensive effect, in addition to citrus juice and pectin which are well known for their hypolipidemic activities.




Principle

Near-infrared spectroscopy (NIRS) is a method that uses light between the visible and infrared wavelength ranges (approximately 800 – 2500 nm) and measures the absorption of light as a function of wavelength. The near-infrared light that interacts with the sample has specific wavelengths that are absorbed based on certain chemical bonds (e.g. C-H, O-H, S-H, and N-H) being present in the sample.

Applications

Biomedical; Biopharmaceuticals

Procedure

1. Prepare samples.
2. Place the sample in the Near Infrared Spectroscopy.
2. Analyze data and identify samples.

Materials

• Sample: Molecules with C-H, O-H, S-H, and/or N-H bonds (Such as: Proteins, Peptide, Water, Alcohol, Fats, Oils, Hydrocarbons); Volatile Organics; Moisture; Fatty Acids; Solids; Carbohydrate; Liquids, Pastes; Tablets; Capsules, etc.
• Equipment: Near Infrared (NIR) Spectroscopy

Notes

1. NIR is non-destructive, and requires little or no sample preparation.
2. NIR reflectance spectra can be used to quickly determine a material’s properties without altering the sample.
3. NIR can depending on wavelength penetrate up to 20 mm of a sample which makes NIR more effective for solid samples.
4. FTIR spectroscopy is, in principle, very similar to Near Infrared (NIR) spectroscopy, but works at longer wavelengths where the chemical information from the samples is more specific.
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